平菇对完全弗氏佐剂诱导性关节炎大鼠的抗关节炎作用的评价

印度中央邦贾巴尔普尔圣拉姆药学技术研究所研究评估了平菇(佛罗里达栽培种)水醇提取物对完全弗氏佐剂诱导的大鼠关节炎模型的抗关节炎作用。

印度中央邦贾巴尔普尔圣拉姆药学技术研究所研究评估了平菇(佛罗里达栽培种)水醇提取物对完全弗氏佐剂诱导的大鼠关节炎模型的抗关节炎作用。通过大鼠左足跖下表面注射弗氏佐剂建立关节炎大鼠模型。实验动物组口服平菇提取物200mg/kg和400mg/kg剂量,吡罗昔康腹腔注射(4mg/kg),并对其抗蛋白酶、白蛋白变性、热诱导溶血等参数进行了体外实验。21天内口服平菇提取物处理的大鼠足趾体积、关节直径和体重的变化显著减少(p<0.001),对应体内蛋白酶活性和膜稳定性均显著降低(p<0.001)。与关节炎模型对照组相比,该处理还促进大鼠握力活动增加。X射线检查显示关节肿胀减轻。提取物组的组织病理学检查显示关节间隙明显缩小,抗体水平也有所上升。抗氧化指标显示超氧化物歧化酶和过氧化氢酶活性显著提高(p<0.001),平菇(佛罗里达栽培种)提取物在大鼠模型中显示出很强的抗氧化活性。结果表明,平菇(佛罗里达栽培种)提取物含有重要的药理活性成分,具有抗关节炎活性。

关键词:平菇(佛罗里达栽培种),炎症,关节炎,足趾体积,X-射线,药用菌

Evaluation of Antiarthritic Effect of Culinary-Medicinal Oyster Mushroom Pleurotus ostreatus cv. Florida (Agaricomycetes) on Complete Freund's Adjuvant Induced Arthritis in Rats

Ayushi Chourasia1, AnkitaTiwari1, Aditya Ganeshpurkar2, Anupam Jaiswal1, AbhishekShrivastava1, Nazneen Dubey1

1Shri Ram Instituteof Technology-Pharmacy, Jabalpur, M.P., India; 2DrugDiscovery Laboratory, Shri Ram Institute of Technology−Pharmacy, Jabalpur,M.P., India

Abstract: The present study evaluates the antiarthritic effect of hydroethanolic extract of Pleurotus ostreatus cv. Florida, which was tested against adjuvant induced arthritis in rat models. Arthritis was induced by administration of complete Freund's adjuvant into the subplantar surface of left paw of rats. The extract was given orally at doses 200 mg/ kg and 400 mg/kg and piroxicam was administered intraperitonially (4 mg/kg). In vitro testing on parameters including antiproteinestrase, albumin denaturation and heat induce hemolysis was also carried out. There was significant decrease (p < 0.001) in proteinase activity and membrane stabilization in vivo studies on cv. Florida extract treated rats showed a significant (p < 0.001) decrease in paw volume, joint diameter, and spontaneous change in body weight recorded for 21 days. The treatment also resulted in an increase in rats' gripping activity compared with arthritic control rats. X-ray examinations showed a decrease in joint swelling. Histopathological examination of the extract treated group showed a significant decrease in joint space. There was also an increase in antibody levels. The antioxidant parameters showed a significant (p< 0.001) increase in superoxide dismutase and catalase enzymatic activities. Thus P. ostreatus cv. Florida extract demonstrates a potent antioxidant activity in a rat model. It is concluded that the P. ostreatus cv. Florida extract contains medicinally important constituents that show antiarthritic activity in rats.

Keywords: Pleurotus ostreatus cv. Florida, inflammation, arthritis, paw volume, X-ray, medicinal mushrooms

原文发表于:International Journal of Medicinal Mushrooms, Volume 21, 2019Issue 11,pages 1123-1136

  • 发表于 2020-03-20 16:24
  • 阅读 ( 69 )
  • 分类:研究前言

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